Journal: Experimental and Therapeutic Medicine

Article Title: Cell sheets of co-cultured BMP-2-modified bone marrow stromal cells and endothelial progenitor cells accelerate bone regeneration in vitro

doi: 10.3892/etm.2019.7982

Figure Lengend Snippet: Characterization of BMSCs and EPCs. (A) Flow cytometry analysis of Ficoll-separated BMSCs surface markers, including CD44, CD71 and CD45. (B) Flow cytometry analysis of Percoll-separated EPCs surface markers, including CD31, CD133 and VEGFR. BMSCs, bone marrow stromal cells; EPCs, endothelial progenitor cells; CD44, CD44 antigen; CD71, transferrin receptor protein 1; CD45, receptor-type tyrosine-protein phosphatase C; CD31, platelet endothelial cell adhesion molecule; CD133, prominin-1; VEGFR, vascular endothelial growth factor receptor; FITC, fluorescein isothiocyanate; PE, phycoerythrin.

Article Snippet: Subsequently, primary antibodies purchased from BIOSS, including fluorescein isothiocyanate/phycoerythrin (FITC/PE)-conjugated rat anti-CD44 (cat. no. bs-4916R-FITC), FITC/PE-conjugated rat anti-CD71 (cat. no. bs-1782R-FITC) and FITC/PE-conjugated rat anti-receptor-type tyrosine-protein phosphatase C (CD45) (cat. no. bs-10599R-FITC) at a concentration of 2 mg/ml for the identification of BMSCs; and FITC/PE-conjugated rat anti-CD31 (cat. no. bs-0195R-FITC), FITC/PE-conjugated rat anti-CD133 (cat. no. bs-0395R-FITC) and FITC/PE-conjugated rat anti-VEGFR (cat. no. bs-0170R-FITC) were added to the cells at a concentration of 2 mg/ml and incubated with anti-mouse compensation beads for 30 min in the dark for the identification of EPCs.

Techniques: Flow Cytometry

Journal: Pharmaceutics

Article Title: Taming the Tumor Stroma: A Two-Stage Targeted Nanocapsule for Potent Deep Chemo-Immunotherapy in Triple-Negative Breast Cancer

doi: 10.3390/pharmaceutics18020184

Figure Lengend Snippet: NPC-ABS/FDS mediated deep chemotherapy and induced immune activation. ( A , B ) Confocal microscopy images in 3D tumor spheres after treated with different groups (a. NPC-FCS, b. NPC-FCS+NPC-AEAA-SLN, c. NPC-FCS+BAE-Sol, d. NPC-FCS+ABS, and e. NPC-ABS/FCS. Z-axis scan spacing = 10 μm. BAE = 2 μg/mL in A and BAE = 5 μg/mL in B, respectively; the magnification was 10×). ( C ) Representative photomicrographs of tumor biopsies treated with different groups (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS). Tumor blood vessels were labeled with FITC-CD31; the magnification was 20×). Relative expression quantity of immune-associated cells in mouse tumors after administration of different preparations of ( D ) CD4 + T cells, ( E ) CD8 + T cells, and ( F ) Tregs (a. normal saline, b. NPC-AEAA-SLN, c. BAE-SLN, d. ABS, and e. NPC-ABS. Mean ± SD, n = 3. ** p < 0.01, *** p < 0.001. Blue color represented positive cells; the magnification was 10×).

Article Snippet: FITC Plus Anti-Mouse CD31 was obtained from Proteintech Group, Inc. (Chicago, IL, USA).

Techniques: Activation Assay, Confocal Microscopy, Saline, Labeling, Expressing

Journal: bioRxiv

Article Title: Defining Cardiac Cell Populations and Relative Cellular Composition of the Early Fetal Human Heart

doi: 10.1101/2021.10.21.465281

Figure Lengend Snippet: (A) Immunohistochemistry of cardiomyocyte markers TnI and α-MHC. (B) Immunohistochemistry of fibroblast markers vimentin, Thy-1 and DDR2. (C) Immunohistochemistry of endothelial and smooth muscle markers, CD31 and α-SMA, respectively. DAPI was used as a counter stair for cell nuclei.

Article Snippet: These included: Myosin Heavy chain (MHC) conjugated to efluor660 (50-6503-80 ebioscience), Troponin I (Ab47003, Abcam), DDR2 (Sc7555 Santa Cruz), Thy-1, (555595 BD Biosciences), Vimentin conjugated to Cy3 (C9080 Sigma), CD31 to APC (21270316 Immunotools) and α-SMA conjugated to FITC (53-9760-80 ebioscience).

Techniques: Immunohistochemistry

Journal: bioRxiv

Article Title: Defining Cardiac Cell Populations and Relative Cellular Composition of the Early Fetal Human Heart

doi: 10.1101/2021.10.21.465281

Figure Lengend Snippet: (A) unstained heart cells, (B) MHC, (C) TnI, (D) Vimentin, (E) DDR2, (F) Thy-1, (G) CD31, (H) α-SMA. Grey peaks in flow cytometry histograms represents isotype/negative controls. (I) Histogram showing the percentage of positive cardiac cells for each marker. Error bars represent standard deviation.

Article Snippet: These included: Myosin Heavy chain (MHC) conjugated to efluor660 (50-6503-80 ebioscience), Troponin I (Ab47003, Abcam), DDR2 (Sc7555 Santa Cruz), Thy-1, (555595 BD Biosciences), Vimentin conjugated to Cy3 (C9080 Sigma), CD31 to APC (21270316 Immunotools) and α-SMA conjugated to FITC (53-9760-80 ebioscience).

Techniques: Flow Cytometry, Marker, Standard Deviation

Journal: bioRxiv

Article Title: Defining Cardiac Cell Populations and Relative Cellular Composition of the Early Fetal Human Heart

doi: 10.1101/2021.10.21.465281

Figure Lengend Snippet: Dual flow cytometry dot plot of TnI and MHC. (B) Gating and colour coding of Vim High (red) and Vim Low (blue) positive cell populations. (C) TnI fluorescence intensity in Vim High and Vim Low cardiac populations. (D) Dual flow cytometry dot plots of vimentin with α-SMA. (E) Dual flow cytometry dot plots of vimentin with CD31. (F) Dual flow cytometry dot plots of vimentin with MHC, populations. Flow cytometry gating based on isotype controls. FSC= forward scatter.

Article Snippet: These included: Myosin Heavy chain (MHC) conjugated to efluor660 (50-6503-80 ebioscience), Troponin I (Ab47003, Abcam), DDR2 (Sc7555 Santa Cruz), Thy-1, (555595 BD Biosciences), Vimentin conjugated to Cy3 (C9080 Sigma), CD31 to APC (21270316 Immunotools) and α-SMA conjugated to FITC (53-9760-80 ebioscience).

Techniques: Flow Cytometry, Fluorescence

Journal: bioRxiv

Article Title: Defining Cardiac Cell Populations and Relative Cellular Composition of the Early Fetal Human Heart

doi: 10.1101/2021.10.21.465281

Figure Lengend Snippet: Dual immunohistochemistry of vimentin with (A) TnI, (B) α-SMA, (C) CD31 and (D) MHC. Asterisks denote blood vessel wall. Arrows identify sarcomeric structures.

Article Snippet: These included: Myosin Heavy chain (MHC) conjugated to efluor660 (50-6503-80 ebioscience), Troponin I (Ab47003, Abcam), DDR2 (Sc7555 Santa Cruz), Thy-1, (555595 BD Biosciences), Vimentin conjugated to Cy3 (C9080 Sigma), CD31 to APC (21270316 Immunotools) and α-SMA conjugated to FITC (53-9760-80 ebioscience).

Techniques: Immunohistochemistry

Journal: bioRxiv

Article Title: Defining Cardiac Cell Populations and Relative Cellular Composition of the Early Fetal Human Heart

doi: 10.1101/2021.10.21.465281

Figure Lengend Snippet: Dual immunohistochemistry of DDR2 with (A) α-MHC, (B) α-SMA and (C) CD31. Myo= myocardium. AO/PA= aorta/pulmonary artery.

Article Snippet: These included: Myosin Heavy chain (MHC) conjugated to efluor660 (50-6503-80 ebioscience), Troponin I (Ab47003, Abcam), DDR2 (Sc7555 Santa Cruz), Thy-1, (555595 BD Biosciences), Vimentin conjugated to Cy3 (C9080 Sigma), CD31 to APC (21270316 Immunotools) and α-SMA conjugated to FITC (53-9760-80 ebioscience).

Techniques: Immunohistochemistry